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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


please help me- im a beginner

please help me- im a beginner - Transfection Forum

please help me- im a beginner - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 01-23-2011, 04:28 PM
Pipette Filler
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Default please help me- im a beginner



Hello all!

I m beginner in cell biology and have to write about cell culture ( mammalian cells culture):

What are the 3 techniques to manipulate mamalian cells?
I know we need a vector system, but what kind of methods do we have?
I only need definitions and short description of this methods...

2) What is a cell culture and the way ( i guess this means steps?) of creating a cell culture?

It s for me very difficult, and i need someone who can good explain such things.

By the way, nice to be here, and hope to get to know you better.

All the best )))

Thanks

Daniela
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Old 02-20-2012, 10:38 AM
Pipette Filler
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Default Re: please help me- im a beginner

Hi HyppGilr,

your questions are very broad and without specifics want experiments you are thinking about, it is very hard to come up with answers.

if you are interested in learning the very basics of cell culture there several resources out there. Some from commercial companies but good nonetheless:

- Sigma's cell culture handkook
- Roche lab FAQS (this one is must to have around in the lab)


Please be more specific and I might help further.
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