Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Transfection Forum
Register Search Today's Posts Mark Forums Read

Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


choosing a method for stable transfection

choosing a method for stable transfection - Transfection Forum

choosing a method for stable transfection - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-16-2010, 03:18 PM
Pipette Filler
Points: 347, Level: 6 Points: 347, Level: 6 Points: 347, Level: 6
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2010
Posts: 7
Thanks: 0
Thanked 0 Times in 0 Posts
Default choosing a method for stable transfection



Hi, I wanted to get a stable transfection in some tumor cells.

I was recommended to take the lentiviral system but I dont know how I can clone my gene of interest into such one. DoI have to take a special vector or does it work with some additional lentiviral packing plasmids???
Reply With Quote
  #2  
Old 02-18-2011, 02:34 PM
Pipette Filler
Points: 169, Level: 3 Points: 169, Level: 3 Points: 169, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2010
Posts: 7
Thanks: 0
Thanked 3 Times in 3 Posts
Default Re: choosing a method for stable transfection

If you can transfect your cells with regular plasmids, do it. Less work and easier to handle. Test with a GFP construct and see if get >5% transfection yield, if yes, go for it.
Reply With Quote
The Following User Says Thank You to Panoramix For This Useful Post:
admin (02-19-2011)
Reply

Tags
choosing , method , stable , transfection


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Transfection method for MEF cell line Burcin Protocols and Methods Forum 0 11-18-2010 10:35 AM
Transfection myoblast laure d'astarac Protocols and Methods Forum 0 09-24-2008 01:55 PM
RNA Kit v.s. Trizol Method c1bl RNA Techniques Forum 5 02-01-2008 08:28 PM
Cylindrical wave, wave equation, and mistakes h_v_ansari@yahoo.com Physics Forum 2 10-26-2006 01:47 AM
Scientific method (was Re: Fusion In Utah (again)) Bill Vajk Chemistry Forum 12 09-29-2003 08:30 PM


All times are GMT. The time now is 10:29 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11651 seconds with 16 queries