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Labie 12-16-2010 03:18 PM

choosing a method for stable transfection
 
Hi, I wanted to get a stable transfection in some tumor cells.

I was recommended to take the lentiviral system but I dont know how I can clone my gene of interest into such one. DoI have to take a special vector or does it work with some additional lentiviral packing plasmids???

Panoramix 02-18-2011 02:34 PM

Re: choosing a method for stable transfection
 
If you can transfect your cells with regular plasmids, do it. Less work and easier to handle. Test with a GFP construct and see if get >5% transfection yield, if yes, go for it.


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