choosing a method for stable transfection
Hi, I wanted to get a stable transfection in some tumor cells.
I was recommended to take the lentiviral system but I dont know how I can clone my gene of interest into such one. DoI have to take a special vector or does it work with some additional lentiviral packing plasmids???
Re: choosing a method for stable transfection
If you can transfect your cells with regular plasmids, do it. Less work and easier to handle. Test with a GFP construct and see if get >5% transfection yield, if yes, go for it.
|All times are GMT. The time now is 08:20 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved