Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Transfection Forum
Register Search Today's Posts Mark Forums Read

Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Pei

Pei - Transfection Forum

Pei - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-24-2010, 01:04 PM
Pipette Filler
Points: 33, Level: 1 Points: 33, Level: 1 Points: 33, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2010
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Pei



Hi!
Has anyone used polyethylenimine (PEI) for transfection? How do you sterilize it? Is it autoclavable? Do you know a company where I can buy it sterile?
Reply With Quote
  #2  
Old 03-20-2012, 08:53 PM
Pipette Filler
Points: 281, Level: 5 Points: 281, Level: 5 Points: 281, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2011
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Pei

Hi,
We use PEI for several years for transfection of 293T. We made 2 mg/ml solution in water, neutralize and filter. Keep at -70C. After maybe 3-4 years stock is still going strong.
Reply With Quote
Reply

Tags
pei


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 02:48 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11122 seconds with 15 queries