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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Double Transfection

Double Transfection - Transfection Forum

Double Transfection - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 03-04-2010, 11:30 AM
Pipette Filler
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Default Double Transfection



I have a problem with transfection efficiency. Therefore I'd like to FACS-sort my transfected cells. My vector of interest, however, has no sortable marker. Would a double transfection, e.g. with a GFP-expression Vector, make sense, or would I get lots of GFP-positive cells that don't have the other vector?
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Old 03-04-2010, 06:03 PM
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Default Re: Double Transfection

Does your vector have a mammalian resistance marker (G418, for example). If so, transfect, let the cells grow for 4-6 hours, then add the antibiotic. Anything not transfected will die.
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Old 03-05-2010, 07:53 AM
Pipette Filler
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Default Re: Double Transfection

In fact it has, but the problem is, the cell line I'm using already is G418 resistant, so that easy way doesn't work for me.
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