I have been trying to transfect HepG2 cells stably via lentiviral transduction and I use puromycin@1microgram/ml for selection. Flow analysis shows that I achieve around 10% transfection efficiency but after transfection, the cells look stressed and do not adhere well to the tissue culture flask. Eventually, I land up losing all the cells. Can anyone give me tips or suggestions to improve transfection efficiency and expand the clones from the population? Any help or pointers will be highly appreciated!
Thanks & Regards