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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


HepG2 Cell Transfection

HepG2 Cell Transfection - Transfection Forum

HepG2 Cell Transfection - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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  #1  
Old 07-18-2009, 05:29 AM
TMU TMU is offline
Pipette Filler
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Default HepG2 Cell Transfection



Hello Experts,

I have been trying to transfect HepG2 cells stably via lentiviral transduction and I use puromycin@1microgram/ml for selection. Flow analysis shows that I achieve around 10% transfection efficiency but after transfection, the cells look stressed and do not adhere well to the tissue culture flask. Eventually, I land up losing all the cells. Can anyone give me tips or suggestions to improve transfection efficiency and expand the clones from the population? Any help or pointers will be highly appreciated!

Thanks & Regards
TMU
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Old 08-11-2009, 02:03 PM
Pipette Filler
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Default Re: HepG2 Cell Transfection

Hi

We've tried various transfection reagents to transfect HepG2 cells, and neither lipofectamine or Calcium phosphate gave good results.

We ended up with ExGen 500 from Fermentas, and it gave us 100% better transfection efficiencies!!!

Good luck!

PhD-C
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Old 10-05-2009, 02:38 PM
Pipette Filler
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Default Re: HepG2 Cell Transfection

Hi TMU !

I've used magnetofection to improve transfection efficiency with less MOI that means that cells were in good shape. You could try viromag or viromag R/L form ozbiosciences.com it works very well.
hope that will help

w. xang
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