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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Cell dies after transient transfection with lipo 2000 on HEK293

Cell dies after transient transfection with lipo 2000 on HEK293 - Transfection Forum

Cell dies after transient transfection with lipo 2000 on HEK293 - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 02-05-2009, 02:55 AM
Pipette Filler
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Unhappy Cell dies after transient transfection with lipo 2000 on HEK293



I'm transfecting DNA plasmids into HEK293 cells with invitrogen lipofectamine 2000.
In last summer, my cells went well with low glucose DMEM and 5% CO2. From this year, our lab changed whole HEK293 cell culture system. We used 11995-high glucose DMEM along with 8% CO2 to grow the cells, and started using Opti-MEM or 11995 free medium to transfect DNA. My cells can grow well with the high glucose DMEM and 8%CO2 W/O transfection. But neither of the Opti-MEM and high glucose DMEM can keep my cells living after transfection with them.
Does anybody have any ideas about this kind of trouble?
Thanks a lot!
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Old 05-17-2009, 09:05 AM
Pipette Filler
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Default Re: Cell dies after transient transfection with lipo 2000 on HEK293

I recently used jetpai, where you can transfect cells in medium. The cells are much happier, but jetpai works best at about 50% confluency.
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