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| Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others. |
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05-19-2008, 10:16 PM
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 Splitting transfected cells ? I am transfecting HepG2 cells with lipofectamine, it takes 72 hours before testing for the expression of GFP for instance, do I subculture the cells within these 72 hours or change the medium or I just leave the cells till the 72 hours end ? Thankss        |
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05-20-2008, 12:25 AM
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| Re: Splitting transfected cells ? Hello, I have worked with HepG2 cells before. I wouldnt subculture them unless you have a stable transfection. Usually we transfected them at 70% confluency so in 3 days they may get crowded. How crowded are they now?  |
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05-20-2008, 06:13 AM
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| Re: Splitting transfected cells ? They are almost 90-100% confluent as am using 35cm plates, what do I do ? Thankss |
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