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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Change Media With Transfection?

Change Media With Transfection? - Transfection Forum

Change Media With Transfection? - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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  #1  
Old 04-18-2008, 02:35 PM
Pipette Filler
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Default Change Media With Transfection?



Hello,
is it a good idea to change the media when you transfect cells with dna and lipofectamine or fugene etc? If so, why is this done?

thanks
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Old 10-27-2008, 07:26 PM
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Default Re: Change Media With Transfection?

Hi,

It is important to change the media because proteins in SFB or the antibiotics may interfere with the lipofectamine, and enter trough the cells membrane along with your DNA, then the efficiency of transfection decrease. But 4 hr affter transfection you must change the medua again... otherwise your cells could die...

I hope it helps
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Old 02-25-2009, 11:06 PM
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Default Re: Change Media With Transfection?

I recently used a transfection reagent called DNAFect LT, which showed excellent performance. There is no need to change medium and no medium change for transfection. You may have a try.
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Old 06-23-2009, 03:19 PM
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Default Re: Change Media With Transfection?

may i know how this DNAFect LT work?
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Old 07-16-2009, 11:44 PM
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Default Re: Change Media With Transfection?

We do change media just before transfection, and if you change 24 h later this would increase your transfection efficiency.
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Old 10-05-2009, 02:54 PM
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Default Re: Change Media With Transfection?

Hi
another alternative is to use magnetofection. After 30 min of incubation of your transfection reagents with cells on a magnetic plate, you can wash your cells (the magnet keeps the transfecting particules at the cell surface while you remove the medium) with fresh medium.
with magnetofection, washing is just an option since the doses are less important thant with other lipid reagents

w.
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Old 12-30-2009, 02:27 AM
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Default Re: Change Media With Transfection?

This topic also is written in wikipedia and about.com.

You can find it by using Google.

Rgs
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Old 07-18-2010, 02:00 PM
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Default Re: Change Media With Transfection?

Yeah, it is better to keep your happy and to increase the transfection efficiency. Therefore you must to change the media 4 hrs post transfection with lipofectamine.
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