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|Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.|
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i have a transfection problem sorry to put it on everyone
My cells were almost 100% confluence before transfection and i used the transfection regeant Lipofetamine 2000
I didnt get much expression of GFP from my construct...
Re: Transfection Problem!
there may be several reasons your transfection failed:
1) 100% confluence is not the ideal situation for transfection - have you tried 60-75%?
2) did you optimize DNA/Lipofetamine 2000 ratios?
3) how is the DNA/plasmid quality? have you been able to express GFP previously?
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