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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Transfection Problem!

Transfection Problem! - Transfection Forum

Transfection Problem! - Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


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Old 04-09-2008, 03:08 PM
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Question Transfection Problem!



Hiya all,
i have a transfection problem sorry to put it on everyone


My cells were almost 100% confluence before transfection and i used the transfection regeant Lipofetamine 2000

I didnt get much expression of GFP from my construct...

Any ideas?
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Old 04-16-2008, 09:15 AM
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Cool Re: Transfection Problem!

Hello

there may be several reasons your transfection failed:

1) 100% confluence is not the ideal situation for transfection - have you tried 60-75%?

2) did you optimize DNA/Lipofetamine 2000 ratios?

3) how is the DNA/plasmid quality? have you been able to express GFP previously?
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