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Transfection Forum Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.


Transfection of Cos-7 Cells

Transfection Forum

Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.



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  #1 (permalink)  
Old 12-28-2007, 10:48 PM
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Default Transfection of Cos-7 Cells
Hello guys,
how can you transfect large DNA 10kb plus plasmids into Cos-7 Cells?

thanks!
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  #2 (permalink)  
Old 12-28-2007, 10:49 PM
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Default Re: Transfection of Cos-7 Cells
Hello Admin!
happy holidays!


Have you tried regular Fugene?
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Old 12-28-2007, 11:06 PM
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Default Re: Transfection of Cos-7 Cells
I have and I get low transfection efficiency any ideas?

cheers
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Old 12-28-2007, 11:16 PM
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Default Re: Transfection of Cos-7 Cells
Have you tried calcium phosphate?

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Old 12-28-2007, 11:19 PM
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Default RE: Transfection of Cos-7 Cells
I have tried calcium phosphate and electroporation for difficult and large
plasmids.

> Have you tried calcium phosphate?
>
>
>
>
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