When is the Best Time to Transfect Cells?

dnamolecule · #1 (**permalink**) 11-09-2007, 01:51 PM

Hi,
When is the Best Time to Transfect Cells?

Does it depend on confluence, cell cycle, age of cells?

thanks

Alyaa Mahmoud · #2 (**permalink**) 04-11-2008, 05:56 AM

Hey
I am very new to cell cultures and transfection but I know that the best time for transfection of any cell line is the Log phase, this would be when the cells are like 70-80% confluent and the number of passages should be less than 50X (for optimum results).
You should also worry about the ratio of the DNA to the transfection reagent u r using.
Hope it helps : )
Good luck

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When is the Best Time to Transfect Cells?

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Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.