Hi, I am trying to differentiate MSCs isolated from human amnion (and amniotic epithelial cells too) into adipocytes and osteoblasts. Cells were plated in 12-wells plates at a density of 25x103 /cm2 for adipogenic differentiation, and 15x103 /cm2 for osteogenic differentiation. After 3 days cells grown to confluency, and i am afraid it is too quickly. My question is: can i passage cells during differentiation? There is 18 days more, and the each well is completely overgrown. I am afraid that cells will start to detach from the bottom and cells begin to die. What should i do?
Thanks for your help.