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#1
10-14-2012, 05:34 PM
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 Mesenchymal stromal cells - differentiation Hi, I am trying to differentiate MSCs isolated from human amnion (and amniotic epithelial cells too) into adipocytes and osteoblasts. Cells were plated in 12-wells plates at a density of 25x103 /cm2 for adipogenic differentiation, and 15x103 /cm2 for osteogenic differentiation. After 3 days cells grown to confluency, and i am afraid it is too quickly. My question is: can i passage cells during differentiation? There is 18 days more, and the each well is completely overgrown. I am afraid that cells will start to detach from the bottom and cells begin to die. What should i do? Thanks for your help.  |
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| cells , differentiation , mesenchymal , stromal |
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