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Low Homologous Recombination Rates Stem Cells

Low Homologous Recombination Rates Stem Cells - Stem Cell Forum

Low Homologous Recombination Rates Stem Cells - Stem Cells and Stem Cell Culture. Embryonic Cell Forum and Mouse Transgenic Forum. Blastocyst injection, ES Cell Selection.


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Old 04-16-2008, 09:04 AM
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Exclamation Low Homologous Recombination Rates Stem Cells



Hello, i am new to the gene targeting gane and to this amazing forum also

I am conducting gene targeting experiments using mouse embyronic ES stem cells, G418 positive and DT negative selection.

My problem is the rate of homologous recombinant clones which actually survive is quite low ~1:500


Anyone have suggestions to increase this frequency?
thanx
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Old 07-23-2008, 12:19 PM
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Default Re: Low Homologous Recombination Rates Stem Cells

hello there wex,
human cells have a very low frequency of homologous recombination let alone stem cells. have you tried to use viral / bacterial selection methods for homologous recombination?
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