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Low Homologous Recombination Rates Stem Cells
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Stem Cells and Stem Cell Culture. Embryonic Cell Forum and Mouse Transgenic Forum. Blastocyst injection, ES Cell Selection.
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04-16-2008, 09:04 AM
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 Low Homologous Recombination Rates Stem Cells Hello, i am new to the gene targeting gane and to this amazing forum also I am conducting gene targeting experiments using mouse embyronic ES stem cells, G418 positive and DT negative selection. My problem is the rate of homologous recombinant clones which actually survive is quite low ~1:500 Anyone have suggestions to increase this frequency? thanx        |
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