culture of mesenchymal stem cells

[rajya]

Dear friends,
Please give me protocols for isolation of mesenchymal stem cells from human bone marrow.
Thanking you,
Rajyalakshmi.

[rajya]

Also give me details about invitro growth of bone with MSC culture.

[moleculardude]

Hello,

I just read up on culture:

mesenchymal stem cells usually are grown in a modified DMEM medium with specific trace elements, FBS (fetal bovine serum) and L-glutamine.

Long-term cultures of MSC may be established without HEPES, however HEPES buffered media was shown to be helpful.

For differentiation of MSC into other lineages such as bone, cartilage, muscle or fat cells, you must add cytokines to media.

Some very nice FAQ was prepared here: FAQ

Please post back what you have found here so we may all learn,
thanks
Cheers!

[rajya]

Dear Dude,
I appreciate your reply.Thank you very much.Why don't you send a few protocols as attachments?
Best Regards,
Rajyalakshmi.

[That_guy_over_there]

There's a whole site in addition to a FAQ, where you can find protocols:hp-h.com/p/murinemsc/

Greetings,

This guy over here.