Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Signalling Biology
Register Search Today's Posts Mark Forums Read

Signalling Biology Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


Phosphatase Inhibitors

Phosphatase Inhibitors - Signalling Biology

Phosphatase Inhibitors - Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-19-2006, 07:53 AM
molecule2005's Avatar
Ph.D Doctorate
Points: 4,766, Level: 47 Points: 4,766, Level: 47 Points: 4,766, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2005
Posts: 176
Thanks: 0
Thanked 6 Times in 6 Posts
Question Phosphatase Inhibitors



Hey everyone!

does anyone have a list of good phosphatase inhibitors they use?
please list them here.

Also if you know which phosphatases they inhibit (serine, tyrosine ?) that would be greatly appreciated!


thanks
Reply With Quote
  #2  
Old 01-05-2007, 02:57 PM
tuttu's Avatar
Pipette Filler
Points: 1,675, Level: 24 Points: 1,675, Level: 24 Points: 1,675, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 24
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Phosphatase Inhibitors

HI,

U may chk this link below, its got some info,

[Only registered users see links. ]

cheers
Reply With Quote
  #3  
Old 05-17-2009, 08:53 AM
Pipette Filler
Points: 134, Level: 2 Points: 134, Level: 2 Points: 134, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2009
Location: California
Posts: 14
Thanks: 0
Thanked 5 Times in 5 Posts
Default Re: Phosphatase Inhibitors

The most potent phosphatase inhibitor is Calyculin A.
Reply With Quote
The Following User Says Thank You to Cancerinform For This Useful Post:
échantillon (07-22-2009)
  #4  
Old 02-23-2011, 03:27 AM
Pipette Filler
Points: 52, Level: 1 Points: 52, Level: 1 Points: 52, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2011
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Phosphatase Inhibitors

There are so many phosphatase inhibitors.I don't what inhibitors you want to know.
Actually,Scientists always use phosphatase Inhibitor cocktail set.
I will list all the phosphatase inhibitors which I know.
(1)bpv
(2)calcineurin autoinhibitory peptide
(3)calyculin A
(4)BN82002
(5)NSC 663284
(6)cyclosprin A
(7)DARPP-32
(8)deltamethrin
(9)dephostatin
(10)3,4-dephostatin
Reply With Quote
  #5  
Old 02-17-2012, 05:52 AM
Banned
Points: 29, Level: 1 Points: 29, Level: 1 Points: 29, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2012
Posts: 8
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: Phosphatase Inhibitors

Sodium orthovanadate is the chemical compound Na3VO4 and contains the tetrahedral VO43-. It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue.
Reply With Quote
  #6  
Old 09-05-2012, 03:45 PM
Pipette Filler
Points: 1,777, Level: 25 Points: 1,777, Level: 25 Points: 1,777, Level: 25
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 15
Thanks: 0
Thanked 5 Times in 5 Posts
Default Re: Phosphatase Inhibitors

No one phosphatase inhibitor on its own will inhibit all classes of phosphatases. I use a combination of sodium fluoride, sodium pyrophosphate, sodium beta-glycerophosphate, and sodium orthovanadate to inhibit phosphatases in cell lysates generated from S. cerevisiae.
Reply With Quote
Reply

Tags
inhibitors , phosphatase


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Molecular Therapy of Breast Cancer: Classicism Meets Modernity (newbook) scimedweb Cell Biology and Cell Culture 0 09-05-2009 10:37 AM
Molecular Therapy of Breast Cancer: Classicism Meets Modernity(announcing) scimedweb@mail.com Cell Biology and Cell Culture 1 05-20-2009 02:12 PM
Acid phosphatase Sakkthi subramaniyam Protocols and Methods Forum 1 05-15-2009 04:49 PM
phosphatase removal in RNA samples Simone Marker Protocols and Methods Forum 1 10-23-2008 08:05 PM
ATP EXTRACTION; ATPASE INHIBITORS Martin Protocols and Methods Forum 2 05-10-2004 02:23 PM


All times are GMT. The time now is 07:57 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13580 seconds with 16 queries