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Signalling Biology Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


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Account ceel - Signalling Biology

Account ceel - Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


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Old 01-29-2011, 11:29 PM
Pipette Filler
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Default Account ceel



please how i can account cells?

now I use the COULTER COUNTER
but i want use another method which is easy method

many thanks
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Old 01-31-2011, 07:02 PM
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Default Re: Account ceel

I don't think there is an easier method than ViCell (Beckman coulter) counting. However, you can do what the counter does manually. You just need a 0.4% Trypan blue solution (prepared in PBS). Here is a link with the protocol:

[Only registered users see links. ]

And here is the protocol of how to count cells on the hemacytometer (just in case you need it):

[Only registered users see links. ]

Hope it can be useful to you.
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