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Signalling Biology Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


Quantification of (poly-)Ubiquitination

Quantification of (poly-)Ubiquitination - Signalling Biology

Quantification of (poly-)Ubiquitination - Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


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Old 07-06-2009, 02:07 AM
Pipette Filler
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Default Quantification of (poly-)Ubiquitination



Hi,

I would like to know if there is a method quantify/assess the degree of ubiquitination of a protein of interest in tissue lysate?
My idea was to do a co-immunoprecipitation of polyubiquitin and my protein but I am afraid this is not quantitative enough.
Or is there a method to quantify the proteasomal degradation of a protein x in vitro?

I have no experience with this area and would greatly appreciate your help!


Thitus
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Old 02-08-2010, 09:25 PM
Pipette Filler
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Default Re: Quantification of (poly-)Ubiquitination

I don't think there is a direct way to quantify the proteasomal degradation of a protein but you could compare the level of ubiquitination of various proteins.
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