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| Signalling Biology Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here. |
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#1
07-06-2009, 02:07 AM
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 Quantification of (poly-)Ubiquitination Hi, I would like to know if there is a method quantify/assess the degree of ubiquitination of a protein of interest in tissue lysate? My idea was to do a co-immunoprecipitation of polyubiquitin and my protein but I am afraid this is not quantitative enough. Or is there a method to quantify the proteasomal degradation of a protein x in vitro? I have no experience with this area and would greatly appreciate your help! Thitus   |
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#2
02-08-2010, 08:25 PM
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| Re: Quantification of (poly-)Ubiquitination I don't think there is a direct way to quantify the proteasomal degradation of a protein but you could compare the level of ubiquitination of various proteins. |
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| polyubiquitination , quantification |
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