Quantification of (poly-)Ubiquitination
I would like to know if there is a method quantify/assess the degree of ubiquitination of a protein of interest in tissue lysate?
My idea was to do a co-immunoprecipitation of polyubiquitin and my protein but I am afraid this is not quantitative enough.
Or is there a method to quantify the proteasomal degradation of a protein x in vitro?
I have no experience with this area and would greatly appreciate your help!
Re: Quantification of (poly-)Ubiquitination
I don't think there is a direct way to quantify the proteasomal degradation of a protein but you could compare the level of ubiquitination of various proteins.
|All times are GMT. The time now is 05:50 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved