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-   -   Quantification of (poly-)Ubiquitination (http://www.molecularstation.com/forum/signalling-biology/69126-quantification-poly-ubiquitination.html)

Thitus 07-06-2009 02:07 AM

Quantification of (poly-)Ubiquitination
 
Hi,

I would like to know if there is a method quantify/assess the degree of ubiquitination of a protein of interest in tissue lysate?
My idea was to do a co-immunoprecipitation of polyubiquitin and my protein but I am afraid this is not quantitative enough.
Or is there a method to quantify the proteasomal degradation of a protein x in vitro?

I have no experience with this area and would greatly appreciate your help!


Thitus:)

prashmi 02-08-2010 09:25 PM

Re: Quantification of (poly-)Ubiquitination
 
I don't think there is a direct way to quantify the proteasomal degradation of a protein but you could compare the level of ubiquitination of various proteins.


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