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-   -   how to test the components of golgi-plasma membrane pathway involved?b (http://www.molecularstation.com/forum/signalling-biology/66428-how-test-components-golgi-plasma-membrane-pathway-involved-b.html)

Anastasia_S 05-29-2009 12:07 PM

how to test the components of golgi-plasma membrane pathway involved?b
 
Hi all,
I'm pretty new in the cell-signaling field so I need help.
I have determined that my chemical downregulates the fully glycosylated membrane version of a receptor, but not the partially glycosylated precursor (which is apparently in the Golgi, a speculation based on a few papers).

How do I attempt to investigate the actual components of the pathway involved?
I work with a mammalian system, non-immortal cells. There are a couple of cell models but the pathway may not be identical.

Please, any idea will help


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