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how to test the components of golgi-plasma membrane pathway involved?b Hi all, I'm pretty new in the cell-signaling field so I need help. I have determined that my chemical downregulates the fully glycosylated membrane version of a receptor, but not the partially glycosylated precursor (which is apparently in the Golgi, a speculation based on a few papers). How do I attempt to investigate the actual components of the pathway involved? I work with a mammalian system, non-immortal cells. There are a couple of cell models but the pathway may not be identical. Please, any idea will help |
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