I don't know the answer to your question concerning dialyzed serum--- I take a shot at it below anyway--- but regarding the general topic of ATP depletion and translocation into the nucleus, there are not all that many publications on that but I have one for you that discusses ATP depletion, translocation and consequences for apoptosis/necrosis (you asked for pitfalls).
Makoto R. Hara, Nishant Agrawal, Sangwon F. Kim, Matthew B. Cascio, Masahiro Fujimuro, Yuji Ozeki, Masaaki Takahashi, Jaime H. Cheah, Stephanie K. Tankou, Lynda D. Hester, Christopher D. Ferris, S. Diane Hayward, Solomon H. Snyder and Akira Sawa. S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding. Nature Cell Biology, 7(7):665–674, 2005
I have not yet made five posts, and so the moderator has anti-spamming policies that don't permit me to give a URL here. Just Google the entire title, and click at the right of the first link that comes up, where it says "all 8 versions". Then click on the 5th one down (counting the indented one), the one from neuroscience_jhu_edu.
I hope that you find the article to be helpful.
Regarding the dialized serum, I found the following from HyClone to be informative:
Dialyzed FBS is used in applications requiring serum depleted of small molecules (less than 10,000 mw) and is filtered through three sequential 100nm (0.1 µm) pore-size rated filters. HyClone has developed a diafiltration process that reduces concentrations of low molecular weight components such as nucleotides and amino acids that are necessary for alternative biochemical survival path ways. The process is reproducible and reduces hypoxanthine and thymidine concentrations below detectable limits. While producing the same results, a major difference between diafiltration and dialysis is that the driving force for diafiltration is hydrostatic pressure, rather than concentration gradients with dialysis. Monitoring glucose concentrations carefully controls the diafiltration process. The glucose concentration, which is representative of the extent of dialysis, is reported for each lot of serum.
Again, this time Google "dialyzed serum definition" and HyClone is the third link down.