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| Signalling Biology Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here. |
Shift of phospho/phospho antibodies
Signalling Biology
Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.
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09-01-2007, 04:03 PM
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 Shift of phospho/phospho antibodies Hi everybody, does someone know a good way to show a phospho-shift in the signal transduction? I mean shall I treat the lysate with a phosphatase and load on to the SDS-PAGE (enzyme-treated vs untreated) or is it sufficiant to incubate with phospho specific abs? Additionally I have just found antibody against tyr. What about serine and threonine? Does anybody know good antibodies? Thanks for any comments!! Tiffy        |
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