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Signalling Biology Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


Interactome for protein protein interactions

Interactome for protein protein interactions - Signalling Biology

Interactome for protein protein interactions - Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.


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  #1  
Old 06-26-2006, 06:19 PM
Pipette Filler
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Question Interactome for protein protein interactions



Does anyone have any experience analyzing protein-protein interactions at the interactome level?

Are you using reversible cross-linking proteomics and mass spectrometry analysis to do this?

are there any other methods for this?

thank u

Ciao!
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  #2  
Old 04-24-2011, 11:23 AM
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Default Re: Interactome for protein protein interactions

Tandem affinity purification is a good technique in analyzing p-p interactions, it is simple to execute.
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Old 07-20-2011, 06:50 AM
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Default Re: Interactome for protein protein interactions

I second that. Here is a beautifully executed paper looking at protein-protein interactions of virtually all yeast kinases.

[Only registered users see links. ]

Doug P
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Old 02-12-2012, 01:57 AM
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Default Re: Interactome for protein protein interactions

At proteinguru.com there is an online software that helps you analyze the mass spec data.
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Old 01-15-2013, 11:02 AM
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Old 01-15-2013, 11:09 AM
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