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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


sds page gel shrinks when casting

sds page gel shrinks when casting - SDS-PAGE Gel Electrophoresis Forum

sds page gel shrinks when casting - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 04-24-2012, 03:54 PM
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Default sds page gel shrinks when casting



Hi, I'm trying to cast my own gels for sds-page, but my gel shrinks when I cast it. For example, I follow all casting instructions for a 7%T gel, pour the solution all the way to the top of the gel cassette, then insert the comb. But 1 hour later, the gel has shrunk to the point where it is below the comb.

Can anyone think of why I would be having this problem? It makes sense that the gel should shrink a little while polymerizing, but not to the point that I'm seeing it. Maybe my acrylamide is old?

Thanks,
Brandon
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Old 04-25-2012, 04:20 AM
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Default Re: sds page gel shrinks when casting

Can you list out your formula for us to have a look? and is your APS freshly prepare?
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