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|SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.|
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smile bands after SDS-PAGE!!!! I'm disperated!!!
I have a problem with my Western blots and I'm getting crazy!!!!
I've already done many western before now and I've always gotten good results but now my gels run very slowly and I have smile bands!!!!! I don't know the reason of these results because I'm using the same conditions of the past!!!!!
I use 15 mA for the stacking and 25 mA for the running gel with a variable voltage like around 300 V.
Could you help me please????
Thank you so much!!!!
Re: smile bands after SDS-PAGE!!!! I'm disperated!!!
don't run the gels with empty wells
Load only sample buffer (1/10th volume of the sample volume) to the blank
|bands , disperated , sdspage , smile|
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