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| SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine. |
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#1
09-24-2011, 11:41 AM
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 smile bands after SDS-PAGE!!!! I'm disperated!!! Hi all!!! I have a problem with my Western blots and I'm getting crazy!!!! I've already done many western before now and I've always gotten good results but now my gels run very slowly and I have smile bands!!!!! I don't know the reason of these results because I'm using the same conditions of the past!!!!! I use 15 mA for the stacking and 25 mA for the running gel with a variable voltage like around 300 V. Could you help me please???? Thank you so much!!!!  |
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#2
10-15-2011, 08:11 PM
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| Re: smile bands after SDS-PAGE!!!! I'm disperated!!! Run the gel with contact voltage |
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#3
10-15-2011, 08:15 PM
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| Re: smile bands after SDS-PAGE!!!! I'm disperated!!! don't run the gels with empty wells Load only sample buffer (1/10th volume of the sample volume) to the blank |
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| bands , disperated , sdspage , smile |
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