Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Basic Lab Protocols and Techniques > SDS-PAGE Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


smile bands after SDS-PAGE!!!! I'm disperated!!!

smile bands after SDS-PAGE!!!! I'm disperated!!! - SDS-PAGE Gel Electrophoresis Forum

smile bands after SDS-PAGE!!!! I'm disperated!!! - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-24-2011, 11:41 AM
Pipette Filler
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default smile bands after SDS-PAGE!!!! I'm disperated!!!



Hi all!!!

I have a problem with my Western blots and I'm getting crazy!!!!

I've already done many western before now and I've always gotten good results but now my gels run very slowly and I have smile bands!!!!! I don't know the reason of these results because I'm using the same conditions of the past!!!!!

I use 15 mA for the stacking and 25 mA for the running gel with a variable voltage like around 300 V.

Could you help me please????


Thank you so much!!!!
Reply With Quote
  #2  
Old 10-15-2011, 08:11 PM
Pipette Filler
Points: 255, Level: 5 Points: 255, Level: 5 Points: 255, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2011
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: smile bands after SDS-PAGE!!!! I'm disperated!!!

Run the gel with contact voltage
Reply With Quote
  #3  
Old 10-15-2011, 08:15 PM
Pipette Filler
Points: 255, Level: 5 Points: 255, Level: 5 Points: 255, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2011
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: smile bands after SDS-PAGE!!!! I'm disperated!!!

don't run the gels with empty wells

Load only sample buffer (1/10th volume of the sample volume) to the blank
Reply With Quote
Reply

Tags
bands , disperated , sdspage , smile


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
sds page faint bands vishwanathiari SDS-PAGE Gel Electrophoresis Forum 3 02-12-2013 02:08 PM
How to Troubleshoot faint SDS-PAGE Gel bands? vishwanathiari SDS-PAGE Gel Electrophoresis Forum 0 09-12-2011 09:16 AM
Troubleshoot Faint SDS-Page Bands with Blue Dye in Lane? vishwanathiari Molecular Biology Techniques 0 09-08-2011 05:44 AM
Convex shape of sample bands on SDS PAGE gel ssacket1 Western Blot Forum 4 12-04-2007 01:09 AM
FS: Old Physics-Related Books Oldbooks78 Physics Forum 0 08-27-2003 02:32 PM


All times are GMT. The time now is 04:28 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12857 seconds with 16 queries