Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   SDS-PAGE Gel Electrophoresis Forum (http://www.molecularstation.com/forum/sds-page-gel-electrophoresis-forum/)
-   -   How to Troubleshoot faint SDS-PAGE Gel bands? (http://www.molecularstation.com/forum/sds-page-gel-electrophoresis-forum/83531-how-troubleshoot-faint-sds-page-gel-bands.html)

vishwanathiari 09-12-2011 09:16 AM

How to Troubleshoot faint SDS-PAGE Gel bands?
 
1 Attachment(s)
I am unable to get good bands in sds page i use 5% stacking gel and 12 % speparting gel, sample was denatured for 10 min at 90 oC satined in comassin blue for 30 min and destained overnight i have attached phot of gel 1st and last lane are markers lane 1,2,3,5,6,7 are protein samples 20 micro liters was loaded in biorad mini conatining 4x sample buffer diluted there is continues blue background in the lane i dont know why its is happening i ran at 60 volts constant for 3hrs aprox
please help me thank you
[Only registered and activated users can see links. Click Here To Register...]


All times are GMT. The time now is 12:05 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08868 seconds with 11 queries