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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


faint bands with blue background in lane

faint bands with blue background in lane - SDS-PAGE Gel Electrophoresis Forum

faint bands with blue background in lane - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 09-08-2011, 06:19 AM
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Default faint bands with blue background in lane



I am unable to get good bands in sds page i use 5% stacking gel and 12 % speparting gel, sample was denatured for 10 min at 90 oC satined in comassin blue for 30 min and destained overnight i have attached phot of gel 1st and last lane are markers lane 1,2,3,5,6,7 are protein samples 20 micro liters was loaded in biorad mini conatining 4x sample buffer diluted there is continues blue background in the lane i dont know why its is happening i ran at 60 volts constant for 3hrs aprox
please help me thank you
kindly see the attachment
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