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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


SDS-PAGE Problems: I'm getting desperate!

SDS-PAGE Problems: I'm getting desperate! - SDS-PAGE Gel Electrophoresis Forum

SDS-PAGE Problems: I'm getting desperate! - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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  #11  
Old 01-29-2011, 09:49 AM
Pipette Filler
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Default Re: SDS-PAGE Problems: I'm getting desperate!

Dear friend,
I had the same kind of problem few months back but later i could figure it out...I had been using tris-Cl for buffer preparation instead of tris free base...I think your Gel pics show me the exact diffused anomalous pattern as mine...TrisCl, Trizma etc results in high concentration of salts giving such weird gels....Good luck
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Old 02-17-2011, 08:03 PM
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Default Re: SDS-PAGE Problems: I'm getting desperate!

Not sure if I made this clear before, but I am certain that the problem I had was that my I wasn't letting my stacking gel set for long enough. I usually wait 30 minutes or more now and haven't had a single problem since.
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