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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Sds page band problem

Sds page band problem - SDS-PAGE Gel Electrophoresis Forum

Sds page band problem - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 11-18-2010, 05:40 AM
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Default Sds page band problem



Dear All,

Suggest me, why I m not getting Band or if at all getting will be then streak or fainted one.

I have attached the Gel Pix, kindly comment on the causes of the gel band pattern.


while running the tracking dye was moving unevenly-any suggestion or reason

Thanks

Arun
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Old 11-18-2010, 05:13 PM
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Default Re: Sds page band problem

Dilute the proteins, I think you have some salts in your samples (a good amount).
You may be overloading the gel, especially in those affected lanes.

Run at constant voltage only, don't limit amps. Start at high voltage for your equipment, about 2/3 of the way, if the skewed pattern is occuring lower the voltage and continue run, the skewed pattern evens out; I do this all the time.
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