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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


SDS PAGE: Pre run

SDS PAGE: Pre run - SDS-PAGE Gel Electrophoresis Forum

SDS PAGE: Pre run - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 10-25-2010, 11:07 AM
Pipette Filler
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Post SDS PAGE: Pre run



dear all,
can somebody tell me is it necessary to pre run the gel in running buffer before loading the sample.if it is to be done what is its purpose.
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Old 03-10-2011, 12:15 PM
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Default Re: SDS PAGE: Pre run

Hello Prachigptgene,

No it is not necessary to pre-run SDS-PAGE gels prior to running the samples. Simply fill the inner and outer buffer reservoirs, load your samples in the lanes, and run.
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Old 03-11-2011, 12:31 AM
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Default Re: SDS PAGE: Pre run

Hi there,
Pre-running of an acrylamide gel was necessary in DNA sequencing gels to get the temperature up and reduce the smiling fronts of bands ("smiley bands").

It is not necessary in discontinuous gel (stacking & resolving) SDS-PAGE gels
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