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| SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine. |
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#1
10-25-2010, 11:07 AM
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 SDS PAGE: Pre run dear all, can somebody tell me is it necessary to pre run the gel in running buffer before loading the sample.if it is to be done what is its purpose.  |
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#2
03-10-2011, 11:15 AM
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| Re: SDS PAGE: Pre run Hello Prachigptgene, No it is not necessary to pre-run SDS-PAGE gels prior to running the samples. Simply fill the inner and outer buffer reservoirs, load your samples in the lanes, and run. |
| The Following User Says Thank You to proteaHeather For This Useful Post: | ||
admin (03-10-2011)
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#3
03-10-2011, 11:31 PM
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| Re: SDS PAGE: Pre run Hi there, Pre-running of an acrylamide gel was necessary in DNA sequencing gels to get the temperature up and reduce the smiling fronts of bands ("smiley bands"). It is not necessary in discontinuous gel (stacking & resolving) SDS-PAGE gels |
| The Following User Says Thank You to admin For This Useful Post: | ||
luisillo (03-11-2011)
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| page , pre , run , sds |
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