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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Blurry Bands on my silver stains!

Blurry Bands on my silver stains! - SDS-PAGE Gel Electrophoresis Forum

Blurry Bands on my silver stains! - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 09-22-2010, 03:23 PM
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Default Blurry Bands on my silver stains!



I can't figure out why I keep getting really blurry bands after developing my 7% silver stain gels. I am running them at low voltages. Any ideas why this might be? Thanks!
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Old 10-12-2010, 09:38 AM
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Default Re: Blurry Bands on my silver stains!

Hallo,
7% silver stain...is it denatured or non denatured and i use 180 v for mini gels of 10% PAA-UREA 7M with silver staining..it works perfectly..try to increase the voltage (which may not be the case)..increase silver concentration, or change your kit.
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