Hi all, recently I ordered some gradient gels from BioRad, and I noticed that when I am running samples the dye smears and forms weird shape (like an upside down smile) especially at the higher gradient conc., is this normal for gradient gels or because of my running conditions. I use 1X Tris-Glycine running buffer w/ SDS (pH not adjusted), cooled, and run my gels in the cold room. I usually set my voltage to 200V and run for 1h. Should I perhaps lower the voltage?
Thanks, any help and suggestiosn would be appreciated