Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Basic Lab Protocols and Techniques > SDS-PAGE Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Transferring Gel to Nitrocellulose Question

Transferring Gel to Nitrocellulose Question - SDS-PAGE Gel Electrophoresis Forum

Transferring Gel to Nitrocellulose Question - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-12-2010, 07:17 AM
Pipette Filler
Points: 6, Level: 1 Points: 6, Level: 1 Points: 6, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Exclamation Transferring Gel to Nitrocellulose Question



On the process of Transferring Gel to Nitrocellulose paper Question...

If you immerse the gel in the transfer buffer (without electrical current running) , Will the proteins in the gel move out in the transfer buffer?

What is the average time you can immerse the gel in transfer buffer (without electrical current running) and keep the protein in the gel?

Will the protein ever move out of the gel into the transfer buffer without the electrical current running?
Reply With Quote
Reply

Tags
gel , nitrocellulose , question , transferring


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Question about work and friction Robert Physics Forum 2 01-04-2008 12:50 PM
Question about Hypothetical Type of Thermal Injury. Explained Radium Protein Forum 13 12-19-2005 09:32 PM
Just a big question... rahemanvelji@yahoo.ca Physics Forum 8 05-09-2005 08:44 PM
Sci.chem FAQ - Part 1 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:06 AM


All times are GMT. The time now is 06:58 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11564 seconds with 16 queries