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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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SDS-page - SDS-PAGE Gel Electrophoresis Forum

SDS-page - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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  #1  
Old 05-03-2010, 06:31 AM
uru uru is offline
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Default SDS-page



Hi. can anyone tell which chemical can replace PMSF in protein extraction buffer?
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Old 07-08-2010, 10:28 AM
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Default Re: SDS-page

dtt i think
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Old 07-08-2010, 10:43 PM
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Default Re: SDS-page

PMSF is serine a protease inhibitor. You can use other kind of protease inhibitor. Or, many companies sell protease inhibitor cocktails that you can use for the same purpose.

DTT is a reducing agent, not really a protease inhibitor.
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Old 07-12-2010, 06:57 AM
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Default Re: SDS-page

ya u r correct! sorry for wrong guess!
i remember know, i use protease inhibitor cocktail (sigma) for my electrophoretic samples and AEBSF(main component of tat mixture) directly for telomerase estimations.
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