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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


12.5% SDS-PAGE gel with vertical lines

12.5% SDS-PAGE gel with vertical lines - SDS-PAGE Gel Electrophoresis Forum

12.5% SDS-PAGE gel with vertical lines - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 04-12-2010, 06:56 AM
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Default 12.5% SDS-PAGE gel with vertical lines



Hi,

I have run many Tris-Gly gels in the past, yet starting to face new problem recently and I don't know what causes it.

A couple of thin vertical lines appeared in my ressolving gel and interferes with the sample run. They are very thin lines (approx 1 mm width), some with few bubbles, but mainly they looked like a groove that goes from bottom to mid/top of ressolving gel.

I don't see the line appear till the gel polymerizes.

I thought it could be a scrach or dirt on the glass plates, yet couln't find anything plus I clean plates with EtOH kimewipe.

No one in my lab cannot figure out what causes it, and I would appreciate if anyone could enlighten me...

Thanks!!
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