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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


SDS-PAGE problem

SDS-PAGE problem - SDS-PAGE Gel Electrophoresis Forum

SDS-PAGE problem - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 12-04-2009, 08:16 PM
Pipette Filler
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Default SDS-PAGE problem



Hi,
I'm running an SDS-PAGE gel in running buffer and Mark 12 standards. the gels are bis-tris gels. every so often the happens to be one well which has a large mass of stained sample at the very top of teh well, where the loading well meets the actual sds-page gel. there is a little streaking down the well sides but other then that the results seem normal. I was wondering if this was a mechanical error on my part in loading the gel. The pippette tip cannot reach the actual gel to puncture it b/c it is too wide and stops after a certain point. Any ideas?
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