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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


proteins not running

proteins not running - SDS-PAGE Gel Electrophoresis Forum

proteins not running - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 11-06-2009, 05:23 PM
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Default proteins not running



Hello. I'm having a problem with proteins getting "stuck" at the top of the gel and not running correctly. This is a new problem in the past month. Previously the gels were running fine. The blue band runs normally to the bottom of the gel and the marker runs fine, but when I blot, the proteins are in a narrow smear toward the top of the gel. We make our own bis/acrylamide gels. Any suggestions?
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Old 11-10-2009, 02:03 PM
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Default Re: proteins not running

Have you tried making the running buffer again? Maybe you forgot something? An undergrad in our lab had a similar problem, and he was just trying to run the gels in 1% SDS, or something like that.
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