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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Fisher EZ-Run restained ladder blurry

Fisher EZ-Run restained ladder blurry - SDS-PAGE Gel Electrophoresis Forum

Fisher EZ-Run restained ladder blurry - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 09-11-2009, 07:01 PM
Pipette Filler
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Default Fisher EZ-Run restained ladder blurry



Hi,

Upon running my gels, my ladder is extremely blurry. I'm using Fisher's EZ-Run pre-stained protein marker, if I use a himark ladder (with high molecular weight markers), my resolution is fine.

I have checked the pH of my upper and lower gel buffers and both are bang on.

Also, my samples themselves run great! I get nice, well defined bands, it's just my ladder.

Does anyone have any ideas where my problem might be?

Thanks!
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