I am running the gels for the Western Blot.
Usually, I combine the volume of my sample that has 20 ug of protein with double volume of buffer, then heat the samples, then centrifuge them and load into wells.
I find it hard to get every little drop out of the tube when I load samples, and I was curious if I can do this:
Instead of mixing, for example 4 ul of sample and 8 ul of buffer, like I would usually do, double my volumes and mix 8 ul of sample and 16 ul of buffer, vortex them, heat, centrifuge, ect,
But when loading pipette 12 ul (e.g the original volume that I was supposed to use).
Thus I donít have to worry that I am leaving stuff on the walls of the tube, and I can rinse the tip of the pipette as well to get the accurate amount.
Will that trick be ok? How do you guys solved this issue for yourself?