I am using Western blot to quantify the relative difference in protein levels between 2 groups (disease and CT), and have n = 8 samples per group (The point is to validate findings from LC/MS). i have run a w blot with all my samples, 8 vs. 8 and find no statistically significnat difference in protein levels, even though my LC/MS experiment did show a difference.
Do you have any suggestions for improving the statistical power of my WB experiment? Unfortunatley i don't have biological replicates yet, so any repeat blots would have to be on technical replicates (ie running those samples samples again). Let me know of any suggestions to increase the statistical power so I might be able to see a statistically signifcant difference.