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Western blot quantification - improving statistical power

Western blot quantification - improving statistical power - SDS-PAGE Gel Electrophoresis Forum

Western blot quantification - improving statistical power - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 07-27-2009, 12:19 PM
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Default Western blot quantification - improving statistical power



Hi guys,
I am using Western blot to quantify the relative difference in protein levels between 2 groups (disease and CT), and have n = 8 samples per group (The point is to validate findings from LC/MS). i have run a w blot with all my samples, 8 vs. 8 and find no statistically significnat difference in protein levels, even though my LC/MS experiment did show a difference.
Do you have any suggestions for improving the statistical power of my WB experiment? Unfortunatley i don't have biological replicates yet, so any repeat blots would have to be on technical replicates (ie running those samples samples again). Let me know of any suggestions to increase the statistical power so I might be able to see a statistically signifcant difference.

cheers
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Old 07-28-2009, 05:30 PM
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Default Re: Western blot quantification - improving statistical power

How about using ELISA?
That's what I do.
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