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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Long Term Storage of SDS-PAGE Gels

Long Term Storage of SDS-PAGE Gels - SDS-PAGE Gel Electrophoresis Forum

Long Term Storage of SDS-PAGE Gels - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 08-26-2008, 10:38 PM
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Lightbulb Long Term Storage of SDS-PAGE Gels



Hi

this is my method to store SDS-PAGE gels for several months:

equilibrate your gels in 4 degrees celcius in two bins containing Transfer Buffer (0.025M tris base,0.192M glycine, 40% MetOH,
10% HOAc - if you are lazy just use plain transfer buffer but will store shorter time) for 15 min.
Pour off excess buffer and freeze the gels.

Carefully thaw gels at room temperature for 30 minutes.
(NOTE DO NOT AGITATE)

Tested with both Biorad mini gels (7cmx10cm) and 16cm x 18cm gels.

Immunostaining, western blot and subsequent blots with HRP and Alkaline phosphatase have worked
consistently even after long storage.


Max Storage: Several months

Last edited by admin; 08-26-2008 at 10:42 PM.
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Old 08-28-2008, 06:34 AM
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Default Re: Long Term Storage of SDS-PAGE Gels

Dear Collegue,
Sounds very interesting! Have you seriously tried the method (as you speak of severl months period of maitanance)? Esp. in terms of display quality, Looking forward to have your comment very soon.
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