| | Re: Choosing the best voltage for SDS-PAGE analysis
there have been several papers indicating that constant current is better especially if you use a stacking gel.
If you are only using a resolving gel then I guess the voltage depends on your protein size and gel percentage. For smaller proteins go a bit faster, for larger go a bit slower.
If you are running your gels on large slabs overnite, I have even used 30V.
For regular gels 100V is ok at room temperature with a cold pack, however faster at 110V or 120V in the cold room with a cold pack should be ok.