Hi, I am having problems after runnning an electroforesis I get really wide bands, compared to the rest in the same gel.
To somewhat illustrate what happens, I load and run my gel and after doing the western we have to quantify the size of the bands to analyse the amount of protein loaded. Most bands come out to a size of 1mm (for example) and then there would be one or two that come out at 1.5mm. The DR says that this is not good for quantifying because they all should be the same or very similar in size.
Now, I know that I don't press too hard on the gel and I am following the protocol exactly, but it still happens. Is it that I am just still pressing too hard? I would appreaciate any help.