SDS Boiling of Samples Any Alternative Methods?

joey20 · #1 (**permalink**) 07-11-2008, 10:46 PM

i hate boiling my samples as i get aggregates of proteins running near the top of the gels. also if you are using specific dyes/probes etc you want hooked to the protein, boiling kills/knocks off these guys.....is there any way to not boil your samples before sds page?

danfive · #2 (**permalink**) 07-14-2008, 07:18 PM

How about native PAGE (non-reducing conditions)?

aftabac · #3 (**permalink**) 07-17-2008, 05:41 AM

So Far i could not find any alternative method of boiling in sample preparation for SDS-PAGE. if somebody know then he should share with us, so we can make a compartive analysis of both procedures.

regards
aftab

keithbrent2000 · #4 (**permalink**) 08-05-2008, 04:28 PM

You could try just not boiling at all to see how the boiled sample compares with the unboiled sample. You could also heat your sample at 37 degrees Celsius for longer (maybe 10 minutes), so that your sample isn't actually boiling. Another possibility could be to include some urea (maybe like 2M) in the sample.

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SDS Boiling of Samples Any Alternative Methods?

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