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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


Centrifuging Samples Prior to Loading Gels

Centrifuging Samples Prior to Loading Gels - SDS-PAGE Gel Electrophoresis Forum

Centrifuging Samples Prior to Loading Gels - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 02-14-2008, 04:44 PM
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Default Centrifuging Samples Prior to Loading Gels



Hello, I have a stupid question

why do we centrifuge samples prior to loading gels such as sds-page gels?
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Old 02-15-2008, 04:49 AM
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Default Re: Centrifuging Samples Prior to Loading Gels

well, it is better to centrifuge your samples prior to loading because if your sample contains some artifacts or other garbage then that settle down and you can load the soluble protein easily, otherwise that also go to the gel while you load your sample and stuck the sample to run in the gel.
so it is better to centrifuge when you have a cell lysate in it, otherwise if you have a purified soluble protein then there is no need of prior centrifugation.

best of luck

regards
aftab
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