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Pipetting Method for Loading Samples onto SDS-Page Gel?

Pipetting Method for Loading Samples onto SDS-Page Gel? - SDS-PAGE Gel Electrophoresis Forum

Pipetting Method for Loading Samples onto SDS-Page Gel? - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 02-14-2008, 04:42 PM
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Arrow Pipetting Method for Loading Samples onto SDS-Page Gel?



Heya,
what do you guys use as a technique to load samples onto an SDS-PAGE gel?
My samples are sometimes lost due to puffing out of the sample.
Any tips or ideas to help?

thanks
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Old 02-15-2008, 04:53 AM
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Default Re: Pipetting Method for Loading Samples onto SDS-Page Gel?

well
there are special tips use to load samples in the Gel and you should use that and try to load sample in a way that you start from the bottom of the well and then gradually move up, in this way hopefully it will stay in the well.
secondly in the gel loading buffer, increase the concentration of glycerol then you use normally, this will make your sample heavy and it will easily go and stay in well.

best of luck

regards
aftab
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Old 08-24-2008, 02:17 PM
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Default Re: Pipetting Method for Loading Samples onto SDS-Page Gel?

Hi

I use Hamilton syringe to load my samples. Its application is very easy.

Best!
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Old 09-09-2008, 03:20 PM
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Default Re: Pipetting Method for Loading Samples onto SDS-Page Gel?

I use specific tips and i load first the sample buffer and then the sample with few drops of glycerol. So if there is to much volume, just the buffer get out and the sample which is heavier fall down in the bottom.
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