SDS-PAGE of Secreted Proteins

[liono]

HIYA!!

IM bak for a question.

I am studying secreted proteins now. My weird question is if I isolate secreted proteins from media, will I have enough of them to run on an sds-page?
I did protein concentration and it seems like there arent many proteins after washing and treating.

I grow the cells for a few hours, day, and days and still low protein.


Also what is the best method to visualize low abundance proteins? Western blot? Coomasie? SYPRO? Silver stain?

thnx guys.

[aftabac]

well
if enough quantity of protein is secreted in the media then you can visulize the protein directly by runing on SDS-PAGE and then by staining with Comassie brilliant blue, if quantity is less then you can stain with silver staining and you can visulize. but if your secreted protein is in very less quantity then you have to concentrate your protein by any of the method, like TCA precipitation or by ammonium sulphate precipitation or any other method and then you cun on SDS-PAGE and can visulize on the gel.
best of luck
regards
aftab