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SDS-PAGE Gel Electrophoresis Forum SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Blurred Distorted Bands SDS-PAGE Gel

SDS-PAGE Gel Electrophoresis Forum

SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.



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  #1 (permalink)  
Old 01-10-2008, 02:45 PM
Pipette Filler
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Unhappy Blurred Distorted Bands SDS-PAGE Gel

Hi, I am having some sds-page gel troubles!

The bands after running are blurred and distorted. How can I fix this what is the problem?

I changed buffers and I still get this...

thanks in advance!
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  #2 (permalink)  
Old 02-04-2008, 12:02 PM
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Default Re: Blurred Distorted Bands SDS-PAGE Gel

Hi
I have had this problem before. I think one reason could be running the gel for too long. It seems that if the tempretur of the tank increases sometimes it makes the protein bands diffuse.
LK
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Old 02-04-2008, 02:52 PM
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Default Re: Blurred Distorted Bands SDS-PAGE Gel

I suggest looking at the Hall of Shame since without a picture I don't have a good idea what the problem with your gel is.
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