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Wicking of Sample - DNA Gel Electrophoresis

Wicking of Sample - DNA Gel Electrophoresis - SDS-PAGE Gel Electrophoresis Forum

Wicking of Sample - DNA Gel Electrophoresis - SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.


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Old 10-30-2008, 11:41 PM
Pipette Filler
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Default Wicking of Sample - DNA Gel Electrophoresis



Hi everyone,

I am having issues with wicking of sample out of gels while I am loading them.

We use reaction volumes of 25uL, with 3uL of 10X Loading Dye. We pipette 18 uL into our gels, leaving a surplus of 7uL unused.

Occasionally (and what seems like randomly), we experience a wicking of the samples out of the wells PRIOR to turning on the gel boxes, and prior to connecting the wires to the power source.

Ideas concerning voltage problems are out, and it seems unlikely that loading dye concentration is the problem, considering the wicking is not reproduceable even within a couple of hours of the problem occuring.

Does anyone have any ideas or suggestions about what may be causing this problem, and how to deal with it!?

Thanks so much!
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Old 10-31-2008, 06:00 AM
Pipette Filler
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Default Re: Wicking of Sample - DNA Gel Electrophoresis

My first guess is Ethanol contaminant of your sample. In my experience, even a tiny amount of residual ethanol (say from a purification or precipitation) causes your sample to diffuse out of the gel. This may also explain why it appears random.

good luck
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dna , electrophoresis , gel , sample , wicking


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